Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.152641 |
Chromosome: | chromosome_11 |
Location: | 330831 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467567 | UBQ1 | Ubiquitin | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGAAGGGCCATCGTTCAAGTTCTGAAACGG |
Internal bar code: | TTTGGCAAGATTGGGCTACAAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 102 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 50 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGGTCGACTCCTTCTGGAT |
Suggested primer 2: | GGCTTGAAATCCCGACTGTA |