Insertion junction: LMJ.RY0402.152642_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.152642
Chromosome:chromosome 2
Location:5480027
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g108000 (1 of 1) K16315 - serine/threonine-protein kinase haspin [EC:2.7.11.1] (GSG2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAGTACGCGTGCACACGCACCTGAACTC
Internal bar code:GGTAAGATCTGAGGGCGGGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1327
LEAP-Seq percent confirming:99.7658
LEAP-Seq n confirming:5538
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGAGGACTGTCCAGAACC
Suggested primer 2:TCCACTGCCTTTTGCTTCTT