| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.152730 |
| Chromosome: | chromosome 1 |
| Location: | 2147803 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011750 | (1 of 1) PTHR31353:SF1 - PROTEIN TAG-322 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAGTCCCATGGTCATGTGAGCCTAACCT |
| Internal bar code: | CGCGGGAGAGAACTTGAATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 170 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGCGACCATAACTCTGC |
| Suggested primer 2: | TGACTGTCAGCTCCGATTTG |