| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.152752 |
| Chromosome: | chromosome 1 |
| Location: | 4798165 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033150 | (1 of 2) PTHR31385//PTHR31385:SF1 - FAMILY NOT NAMED // EXPRESSED PROTEIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTATAGAGAAAACCATTGCCTTCCGTGAC |
| Internal bar code: | AGGACTCTTCGCCCTTCAGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 953 |
| LEAP-Seq percent confirming: | 99.7895 |
| LEAP-Seq n confirming: | 948 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTACGGAAGATGTGTG |
| Suggested primer 2: | CTCATCACTGCACCTCAGGA |