| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.152811 |
| Chromosome: | chromosome 3 |
| Location: | 3916582 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171387 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTTTGCTGCCCCCGAACTAGGCCCGTTC |
| Internal bar code: | ATTGAGATATGTATATCTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 69.3432 |
| LEAP-Seq n confirming: | 9966 |
| LEAP-Seq n nonconfirming: | 4406 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTGTGCTGTTTCTCAAG |
| Suggested primer 2: | TTAAAGCGTGCTGTGAATGG |