Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.152834 |
Chromosome: | chromosome 17 |
Location: | 2082619 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGCCTCCCCTACGCATGCAACACGACA |
Internal bar code: | AGTTGTGTGTCGCGCGTAGAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 288 |
LEAP-Seq percent confirming: | 86.4573 |
LEAP-Seq n confirming: | 881 |
LEAP-Seq n nonconfirming: | 138 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGTGGGATGCGTGTTAGGTC |
Suggested primer 2: | TGGTGCTCTATTGCGAGATG |