| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.152853 |
| Chromosome: | chromosome 3 |
| Location: | 8535652 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201664 | GIT2 | (1 of 1) PF04114 - Gaa1-like, GPI transamidase component (Gaa1); Gaa1-like membrane protein, component of GPI transamidase complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGATGCTGCCAGCAGGGGACCAACTCTA |
| Internal bar code: | CGTGGACCGCCCATCTCTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 98.1806 |
| LEAP-Seq n confirming: | 1457 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGAGAGTGCTGGTGCTG |
| Suggested primer 2: | CCTCCTTAGCTCCGTGACAG |