| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.152855 |
| Chromosome: | chromosome 2 |
| Location: | 3423410 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095111 | VSR1 | Vacuolar Sorting Receptor; (1 of 1) PTHR22765//PTHR22765:SF56 - RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING // VACUOLAR-SORTING RECEPTOR 5-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAACCCCCCACCCACCCCCTGCCTGGCTA |
| Internal bar code: | TAACTATCTTTTAGTCTACTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGAGAGGGGAATGAGGT |
| Suggested primer 2: | TGGCTACGCCTTCACTTCTT |