Insertion junction: LMJ.RY0402.152856_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.152856
Chromosome:chromosome 9
Location:2812277
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g388850 FAP329,ACA1 Flagellar Asociated Protein 329; (1 of 1) PF00122//PF00689//PF00690//PF12710 - E1-E2 ATPase (E1-E2_ATPase) // Cation transporting ATPase, C-terminus (Cation_ATPase_C) // Cation transporter/ATPase, N-terminus (Cation_ATPase_N) // haloacid dehalogenase-like hydrolase (HAD) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCTCAGCACAGGCACTTCGTCAGCCGCG
Internal bar code:GCCGTGCCGCCAATTCCGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:644
LEAP-Seq percent confirming:62.2182
LEAP-Seq n confirming:690
LEAP-Seq n nonconfirming:419
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTGACACGACACTTCCC
Suggested primer 2:TGAGGGTATTTTGAGTGGGC