| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.152856 |
| Chromosome: | chromosome_9 |
| Location: | 2812329 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g388850 | ACA1 | P-type ATPase/cation transporter, plasma membrane | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGTATGCTGGCGAGCGTGGGCTGTACCCC |
| Internal bar code: | CGTCTGGAAGGAGTAGGGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 891 |
| LEAP-Seq percent confirming: | 95.7747 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTGACACGACACTTCCC |
| Suggested primer 2: | TGAGGGTATTTTGAGTGGGC |