Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.152860 |
Chromosome: | chromosome_9 |
Location: | 3324365 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g388393 | ELG13 | Exostosin-like glycosyltransferase | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCCACATGTAGACTGCCACCGGACGGCTCC |
Internal bar code: | GAGTTCGTACGGGCACTGCGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 523 |
LEAP-Seq percent confirming: | 99.5825 |
LEAP-Seq n confirming: | 3101 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCAACGATGCAAAGCACTC |
Suggested primer 2: | TACCTGGCCTCTCCTTCTCA |