| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152862 |
| Chromosome: | chromosome 14 |
| Location: | 880448 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613501 | ZXE5 | (1 of 9) PTHR13789//PTHR13789:SF186 - MONOOXYGENASE // SUBFAMILY NOT NAMED; Putative zeaxanthin epoxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGACGATGGAGGTGAAGATGAGGTGGTGG |
| Internal bar code: | AATTTATAGACACTGTATCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 350 |
| LEAP-Seq percent confirming: | 96.8627 |
| LEAP-Seq n confirming: | 741 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGTATGTGTGTTGGTGC |
| Suggested primer 2: | GCTACTAGCTAACCGCCGTG |