| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.152884 |
| Chromosome: | chromosome 16 |
| Location: | 7720608 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691440 | FAP43 | Flagellar Associated Protein 43; (1 of 2) PTHR14885//PTHR14885:SF1 - UNCHARACTERIZED // WD REPEAT-CONTAINING PROTEIN 96 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCACACGCGACATGCAGGTGAGGCAGG |
| Internal bar code: | GTTACGCTGTGCCCAGCGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 97.548 |
| LEAP-Seq n confirming: | 915 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACCTGTCTGGCCTCAAG |
| Suggested primer 2: | ACAGCCTTGGACCTTCCTTT |