| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152928 |
| Chromosome: | chromosome_14 |
| Location: | 877191 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613450 | CGL87 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGTCAAGGCGTAGGCTTGCAGCGTGTGCG |
| Internal bar code: | GGGTAACCTAGGGGTCGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 51.1312 |
| LEAP-Seq n confirming: | 1582 |
| LEAP-Seq n nonconfirming: | 1512 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTGTCGCTTATGAGTGT |
| Suggested primer 2: | AACATTCGAAACAGAACCCG |