Insertion junction: LMJ.RY0402.152938_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.152938
Chromosome:chromosome 9
Location:5822413
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g402182 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCCCTCTCCCCTCCCCTCTCCACCCCCC
Internal bar code:TGCCCCGGAGCTGACGAATGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:304
LEAP-Seq percent confirming:99.3902
LEAP-Seq n confirming:163
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTAGCTGCCTTGGATG
Suggested primer 2:GTGGACGTTGACATGTGAGG