| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.152961 |
| Chromosome: | chromosome 16 |
| Location: | 517700 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692800 | AKR4 | Aldo/keto reductase; (1 of 1) PTHR11732//PTHR11732:SF284 - ALDO/KETO REDUCTASE // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGAAGAAGGCGGGCAACGTTGGCACCAG |
| Internal bar code: | TCGAACCACCGAGCATGCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.8587 |
| LEAP-Seq n confirming: | 2120 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCAGCTGAGTGACTGAC |
| Suggested primer 2: | GCATGGTTTTGTATTGCGTG |