| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152972 |
| Chromosome: | chromosome 1 |
| Location: | 887063 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004651 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATCACTGATGCTTTCTGTTGTCTCGCTG |
| Internal bar code: | ACGCAGAGATCTCGGGAGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 97.1867 |
| LEAP-Seq n confirming: | 380 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATTTCTGCAATTGTGGGG |
| Suggested primer 2: | GAGCGATTAGGTGAGTTCGC |