| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152976 |
| Chromosome: | chromosome 9 |
| Location: | 1972437 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394850 | TEF24 | LrgB-like protein; (1 of 1) PTHR30249 - PUTATIVE SEROTONIN TRANSPORTER | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTAGGCGTTGCGACTGCCAGGCGTAGG |
| Internal bar code: | CTTGTGGTCACGGGCTATACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 99.6732 |
| LEAP-Seq n confirming: | 915 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTGTGGACTTTGGAGTG |
| Suggested primer 2: | CTAAAGAGGCGCAACTGGTC |