| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.152986 |
| Chromosome: | chromosome 13 |
| Location: | 115066 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g562750 | CGLD38 | Conserved in the Green Lineage and Diatoms; (1 of 1) PF14234 - Domain of unknown function (DUF4336) (DUF4336) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGTTGAAGGTGTGTGTTTTAGCCCCGT |
| Internal bar code: | GCCGGATAGCACGATATGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 90.8333 |
| LEAP-Seq n confirming: | 218 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACTTCACTGGGTTTCCC |
| Suggested primer 2: | ACTAAAGCAAACGGGAGGGT |