| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153024 |
| Chromosome: | chromosome 6 |
| Location: | 1190514 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257500 | FTT2 | 14-3-3 protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTCAGCACTGGTGTCAGCATTCTCGGT |
| Internal bar code: | GCAACCTTGCGATACGAAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 390 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 532 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGTCGGAGCAAGCTAAG |
| Suggested primer 2: | GTATGGGAGGTGTAGCGCAT |