| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153040 |
| Chromosome: | chromosome 3 |
| Location: | 3108040 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164850 | (1 of 5) PF13391 - HNH endonuclease (HNH_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTAAACAATGGCAAGCCCTTGTACACGC |
| Internal bar code: | CCCGTATGGTATGATCAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 146 |
| LEAP-Seq percent confirming: | 99.6479 |
| LEAP-Seq n confirming: | 283 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCATTTATGGCAAAAACA |
| Suggested primer 2: | GTAATCTCCCCCTCCACCAT |