| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.153054 |
| Chromosome: | chromosome 1 |
| Location: | 2126264 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011500 | RPN11 | 26S proteasome regulatory subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGAATGGACTGGAGCGTGTGTGACGAGC |
| Internal bar code: | GACACTATAGCGTGCTTCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1021 |
| LEAP-Seq percent confirming: | 99.6932 |
| LEAP-Seq n confirming: | 7473 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTTGTGTTTGGTCAGTA |
| Suggested primer 2: | TGCACATCGATTTCCGATAA |