| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153083 |
| Chromosome: | chromosome 2 |
| Location: | 1801462 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086887 | NUP98-96,NUP189 | Nucleoporin 98-96; (1 of 1) K14297 - nuclear pore complex protein Nup98-Nup96 (NUP98, ADAR2, NUP116) | 3'UTR|intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACACGGAGCCGCCACCTGCGCACCGGG |
| Internal bar code: | ATTCTCGCGCCGCGCTGTAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 99.6257 |
| LEAP-Seq n confirming: | 1331 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGATGCAAGAAATGGTTTT |
| Suggested primer 2: | GCCGTCCAGTAAAGACAAGC |