Insertion junction: LMJ.RY0402.153097_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.153097
Chromosome:chromosome 8
Location:3919245
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g378300 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGGAAACACCAACACACGGCGGGAGAC
Internal bar code:TCGGCCTCCCTTAAGGTGCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:651
LEAP-Seq percent confirming:48.8636
LEAP-Seq n confirming:172
LEAP-Seq n nonconfirming:180
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTACTCTTCTCACCCGTC
Suggested primer 2:CGCACACACACACACACATT