| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153098 |
| Chromosome: | chromosome 13 |
| Location: | 4084702 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591400 | COA1,COAA1 | (1 of 1) 2.7.1.33 - Pantothenate kinase / Pantothenic acid kinase; Pantothenate kinase, CoA biosynthesis | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGGCCGAAGCGCTGCACCTCGACCCCT |
| Internal bar code: | TCGCTAGCCCATAGTACAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 134 |
| LEAP-Seq percent confirming: | 99.7218 |
| LEAP-Seq n confirming: | 717 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCCTGATTAAGCTCGTG |
| Suggested primer 2: | GACCGTGCACTCCCTACATT |