| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153104 |
| Chromosome: | chromosome 17 |
| Location: | 4903162 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734400 | CUL2,CUL3 | (1 of 1) K03347 - cullin 1 (CUL1, CDC53); Ubiquitin ligase SCF complex subunit Cullin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCACCTTACCCTGTCCAGGCCAGGCCAT |
| Internal bar code: | GCGTCGGGCAACACCTCGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 36 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTAGTTATTTGGCCCTGG |
| Suggested primer 2: | TAAACTTTTGGCAGGGGTTG |