| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.153120 |
| Chromosome: | chromosome 17 |
| Location: | 2781736 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718650 | (1 of 1) K14841 - ribosome biogenesis protein NSA1 (NSA1, WDR74) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTAGGAGGCTTCTTGCAGATGTGGGTGG |
| Internal bar code: | AGTTAATTGACCTGATAAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 78.9474 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACCGGTAGTATGGCTGC |
| Suggested primer 2: | AGGTGTGCACGTGTGTTCAT |