| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153143 |
| Chromosome: | chromosome 3 |
| Location: | 164825 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144204 | (1 of 2) PTHR10219 - GLYCOLIPID TRANSFER PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCTAGGTGGTGGAGTCTGGAGGACAGGT |
| Internal bar code: | GACCAACCACGACCATCAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 840 |
| LEAP-Seq percent confirming: | 99.4798 |
| LEAP-Seq n confirming: | 765 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCATCCATACCTACATGG |
| Suggested primer 2: | ATGCGGTGCTGTGTGTATGT |