| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153147 |
| Chromosome: | chromosome 6 |
| Location: | 4997489 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280000 | non-conserved protein of unknown function | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAGGCAGCCTCTGCAAAAAACCCCATG |
| Internal bar code: | AAGGACGAAGGTTTCAGAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 768 |
| LEAP-Seq percent confirming: | 92.735 |
| LEAP-Seq n confirming: | 1519 |
| LEAP-Seq n nonconfirming: | 119 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTAGCTTCGACTCGCTGT |
| Suggested primer 2: | CGACACTGACTGCCTTGTGT |