| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153158 |
| Chromosome: | chromosome 2 |
| Location: | 6938043 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g120100 | RBCS1 | (1 of 2) K01602 - ribulose-bisphosphate carboxylase small chain (rbcS); Ribulose-1%252C5-bisphosphate carboxylase/oxygenase small subunit 1, chloroplastic | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGTCGACGAGCAAGCGCAGCCCGAAGG |
| Internal bar code: | TTTTCCGGGCGCTGAATTGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 953 |
| LEAP-Seq percent confirming: | 99.6989 |
| LEAP-Seq n confirming: | 2318 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAGACCCATATCCTGGC |
| Suggested primer 2: | ATGGTCCAGTAGCGGTTGTC |