| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.153158 |
| Chromosome: | chromosome_17 |
| Location: | 3366552 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g723600 | IFT81 | Intraflagellar transport protein 81 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CATCCTGCGATGGGTGGTGCCGCAGGCGCA |
| Internal bar code: | ATCAAAACCATTGCCAAGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 639 |
| LEAP-Seq percent confirming: | 99.8446 |
| LEAP-Seq n confirming: | 5140 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATCTGCAGTGAAGTGAA |
| Suggested primer 2: | GAGCTGGGAGTTGACTCTGG |