| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.153167 |
| Chromosome: | chromosome 13 |
| Location: | 645947 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g566000 | FTL1 | Putative monofunctional formate-tetrahydrofolate ligase; (1 of 1) 6.3.4.3 - Formate--tetrahydrofolate ligase / Tetrahydrofolic formylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGATAAGGAACCAGGCGCGCTGCAAGGA |
| Internal bar code: | CCAATGCGCTCGGCTTGATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 99.7319 |
| LEAP-Seq n confirming: | 1488 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCCGTACCACAAGAATG |
| Suggested primer 2: | GGTTGTAGATGCCAGCCCTA |