Insertion junction: LMJ.RY0402.153182_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.153182
Chromosome:chromosome 6
Location:2699077
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g270400 GT90-6,GT90F6 GT90 family protein 6; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGGGAAGGCGCGCGGGGAGAGGTCGAG
Internal bar code:GGTCGTTAAATCACCGGAGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:530
LEAP-Seq percent confirming:99.7326
LEAP-Seq n confirming:746
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ATTTACGCGAGTCAGGGAGA
Suggested primer 2:ACCAGTCCAAGCAATCAACC