| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.153185 |
| Chromosome: | chromosome 12 |
| Location: | 4231968 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518950 | PGM13 | (1 of 2) PTHR23029//PTHR23029:SF12 - PHOSPHOGLYCERATE MUTASE // PHOSPHOMUTASE PMU1-RELATED; Phosphoglycerate mutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTGTCCTGATCTTGCTAGACTTGCTAG |
| Internal bar code: | GCTTTCAAGTGGTACGGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 311 |
| LEAP-Seq percent confirming: | 98.1132 |
| LEAP-Seq n confirming: | 208 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCTTCGACGGTATGGGT |
| Suggested primer 2: | GTTGGGGTAATGCTGCTGTT |