Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.153191 |
Chromosome: | chromosome 9 |
Location: | 6138358 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g405350 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAAAGGGCTTTCTGTGGACACACGCGC |
Internal bar code: | CTCTCGCTCCCTGACGAATCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 918 |
LEAP-Seq percent confirming: | 99.7475 |
LEAP-Seq n confirming: | 1580 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGACTTCACGCGGTTTATGT |
Suggested primer 2: | CGTTTAGCAGGAGCACATGA |