| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153206 |
| Chromosome: | chromosome 12 |
| Location: | 330135 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494600 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCCCGCTGCGTGCCTGCGCTCCGGTCA |
| Internal bar code: | TGACAGAGACGAGTATAACAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 97.454 |
| LEAP-Seq n confirming: | 3177 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGACTGCCCTTTCTTCG |
| Suggested primer 2: | GCAGGAACTGTCATTGGGTT |