| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153208 |
| Chromosome: | chromosome 16 |
| Location: | 4171339 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668850 | A2,MTA2 | Hydroxyproline-rich glycoprotein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACTGAAACGAGGACTGGTGAGTGCTCAG |
| Internal bar code: | TCGTAAGAGCCGGTCTGGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 99.0346 |
| LEAP-Seq n confirming: | 2462 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGGCGGTATCTCTAGG |
| Suggested primer 2: | ACACCATTTTACCGCTCTGG |