| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.153218 |
| Chromosome: | chromosome 14 |
| Location: | 1975320 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621351 | (1 of 2) 2.7.6.5 - GTP diphosphokinase / Stringent factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACGGGGCAGCAGGCGAAAGGTCACGGA |
| Internal bar code: | TTTAACTCCCCCGAAAGCCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 99.4663 |
| LEAP-Seq n confirming: | 1491 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGATCAACACAACAGGATG |
| Suggested primer 2: | GGACTACTGGGTGTGGCTGT |