| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.153237 |
| Chromosome: | chromosome_7 |
| Location: | 945919 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g319150 | PTP4 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTATGGCGAAGTATCAGCGACACACCCGC |
| Internal bar code: | TGCGACGAGCGTGCCGTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 99.4065 |
| LEAP-Seq n confirming: | 1340 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCTTGGAAGCCATCGACT |
| Suggested primer 2: | CATCGAACTGCGTGTGAACT |