| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.153242 |
| Chromosome: | chromosome 3 |
| Location: | 5800317 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188950 | SMM11 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PF07942 - N2227-like protein (N2227) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCTGAGGGTTGCGGCGATGATAGTTTC |
| Internal bar code: | TACCTGCTCGTGAAACGCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 99.5734 |
| LEAP-Seq n confirming: | 2334 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGAAAGCCCTGACTCGT |
| Suggested primer 2: | TCCCTCAACTCCTATGCACC |