| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.153255 |
| Chromosome: | chromosome 17 |
| Location: | 1517629 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707200 | LIC2 | (1 of 5) PF02931 - Neurotransmitter-gated ion-channel ligand binding domain (Neur_chan_LBD); Ligand-gated ion channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGTGGTGAGCCTGGGTGGGGTGGGGG |
| Internal bar code: | CGGGGTACCGACTGGTCAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 350 |
| LEAP-Seq percent confirming: | 99.6346 |
| LEAP-Seq n confirming: | 5726 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGGCATGACTTGTTAGAT |
| Suggested primer 2: | CCTTAGGGCCATAAATGGGT |