| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.153290 |
| Chromosome: | chromosome 17 |
| Location: | 3222615 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722400 | MOT25 | Predicted protein; (1 of 1) PTHR33958//PTHR33958:SF1 - FAMILY NOT NAMED // PROTEIN C8ORF37 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATATGTCGCATTCAACGAGTATCACAGC |
| Internal bar code: | CATGCAACGCCCGTATATTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1073 |
| LEAP-Seq percent confirming: | 97.0564 |
| LEAP-Seq n confirming: | 3594 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATGTCTCGAACTGGGTC |
| Suggested primer 2: | TATAATGCGCTGAGGTGCAG |