| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153292 |
| Chromosome: | chromosome 1 |
| Location: | 7139236 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g051550 | HEL8,SSA9,HEL9 | (1 of 1) PTHR18934:SF148 - ATP-DEPENDENT RNA HELICASE DHX34-RELATED; DEAD/DEAH box helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCCGAATGAGGGACATGTGCACATAAC |
| Internal bar code: | TCGTAAGGCAACCCTCAGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 868 |
| LEAP-Seq percent confirming: | 99.67 |
| LEAP-Seq n confirming: | 1812 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATTTCGGTGGTGTAAGT |
| Suggested primer 2: | GCAGTAGCAAGGGGTGTCAT |