| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153311 |
| Chromosome: | chromosome 1 |
| Location: | 4615351 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031750 | (1 of 1) K11883 - RNA-binding protein NOB1 (NOB1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAGCGGCGAAGGATGACATGAGTTTGG |
| Internal bar code: | TTCTCAGCAGTTGTTTCGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.6438 |
| LEAP-Seq n confirming: | 3916 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGCATAGCATGTAGGAAT |
| Suggested primer 2: | AAACGTGGGAGGGCTAAGTT |