Insertion junction: LMJ.RY0402.153326_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.153326
Chromosome:chromosome 9
Location:2271403
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g392500 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTTGGGCAGTAGGCCGGCATGGAGTGCT
Internal bar code:GATCCGTACATACGTTAAATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:962
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:462
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACATTCTATGATCGCCT
Suggested primer 2:ACTGTCGAGGGCAAGTATGG