| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153339 |
| Chromosome: | chromosome 10 |
| Location: | 2720734 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438250 | LAT2 | Latrunculin-B sensitive 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGTGTGCCGTGGAAGTAAAGGAACGTG |
| Internal bar code: | GAGCGATGGGGATCCTTCGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 99.0937 |
| LEAP-Seq n confirming: | 328 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGAGTGCTCTGTGCTCG |
| Suggested primer 2: | CAAACCTGTTGCGACTCAGA |