Insertion junction: LMJ.RY0402.153348_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.153348
Chromosome:chromosome 7
Location:2188472
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g327000 (1 of 1) PF08217 - Fungal domain of unknown function (DUF1712) (DUF1712) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCAAACCCTAGTGCCGTCGGCACGCTCA
Internal bar code:TTGCAGTAATAGAAATCGGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:681
LEAP-Seq percent confirming:98.194
LEAP-Seq n confirming:1468
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AGCACGAACACGTCGTACAG
Suggested primer 2:GAACTCAGACAGGGGTGGAA