| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.153387 |
| Chromosome: | chromosome 10 |
| Location: | 1588909 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429350 | (1 of 6) 3.4.22.69 - SARS coronavirus main proteinase / Severe acute respiratory syndrome coronavirus main protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGGCATCACAGAGGTGCAGCGTGCACC |
| Internal bar code: | AGCCAGGGGCTACCCCGTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1191 |
| LEAP-Seq percent confirming: | 99.4063 |
| LEAP-Seq n confirming: | 5860 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGCCAGTCCCGACATAC |
| Suggested primer 2: | CCCTAGCTTCGTAGCTCCCT |