| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153440 |
| Chromosome: | chromosome 7 |
| Location: | 6256179 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g356600 | (1 of 1) PF08007 - Cupin superfamily protein (Cupin_4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACAGTGGGCAACCTGCTGGGAGAGGTGA |
| Internal bar code: | TATTCAGCCGGGCGCGGAGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 21.9231 |
| LEAP-Seq n confirming: | 114 |
| LEAP-Seq n nonconfirming: | 406 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | GTGAGGCCGTGCTTGTAGAT |