| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.153458 |
| Chromosome: | chromosome 1 |
| Location: | 3051210 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018750 | DIV155 | (1 of 44) IPR000048 - IQ motif, EF-hand binding site | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATGGACACCGTGCATCAAGAGCTGCGC |
| Internal bar code: | TTCATTGACTATCGCTGATAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 211 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCAGCGAGGAGCAATCT |
| Suggested primer 2: | CTTCTCACCCAGCCATCTGT |